In recent years, trying to detect low level or fast fluorescence signals has proved challenging and an EM-CCD camera was commonly used, however, with the arrival of the ORCA-Flash4.0 that situation has now changed. The ORCA-Flash4.0 is claimed to be the first camera to be able to deliver better signal-to-noise than EM-CCDs, cooled CCDs and first-generation sCMOS cameras for low-level, fast, fluorescence imaging. The ORCA-Flash4.0’s high sensitivity means extreme versatility. This camera covers a wide range of imaging needs, including super resolution microscopy, TIRF microscopy, live cell GFP, high-speed calcium ion imaging, FRET, real-time confocal microscopy and many more.
The ORCA-Flash4.0 has quantum efficiency values of over 70 per cent at 600nm and 50 per cent at 750nm and has only 1.3 electrons of read noise (at 100 full-resolution frames per second) with continuous high speed acquisition at full resolution. This combination of high quantum efficiency and low noise, in the absence of EM-CCD multiplicative noise, means that researchers are pushing the boundaries of what has been possible to image until now.